Preparing and observing a microscope slide | Science | Quizalize & Oak National Academy

Starter quiz

A ______ is a piece of equipment that is used to see things that are very small.
- 'microscope' ✓
Which parts of the microscope magnify an image?
- eyepiece lens ✓
- light
- focusing wheel
- objective lens ✓
Which is not a part of a microscope?
- focusing wheel
- light/mirror
- telescope ✓
- stage
- eyepiece lens
Which action would you take to increase the magnification of this image (to make the individual cells appear larger)?
- move the stage
- increase the brightness of the light
- turn the focusing wheel
- increase the value of the objective lens ✓
Which of these images is more focused?
A ______ is a piece of curved glass that focuses and magnifies light.
- 'lens' ✓

Which piece of apparatus is not used to prepare an onion tissue slide?
- mounted needle
- tweezers
- cover slip
- magnifying glass ✓
Match the apparatus to its job in preparing a microscope slide.
- glass slide
  
  ⇔
  
  put the specimen on ✓
- stain
  
  ⇔
  
  to see the specimen more clearly ✓
- cover slip
  
  ⇔
  
  to place over the specimen ✓
- tweezers
  
  ⇔
  
  to hold the tissue and cover slip ✓
- mounted needle
  
  ⇔
  
  to lower the cover slip slowly ✓
Starting with a glass slide, put these stages in the correct order for preparing a microscope slide.
- 1
  
  ⇔
  
  Take a glass slide and place a tissue or cell specimen on it.
- 2
  
  ⇔
  
  Add a drop of stain.
- 3
  
  ⇔
  
  Place a cover slip over the top using tweezers.
- 4
  
  ⇔
  
  Lower the cover slip with a mounted needle.
- 5
  
  ⇔
  
  Blot off excess stain.
The chemical that is placed on a specimen so that it can been seen easily is called a...
- 'stain' ✓
Which features should be altered in this scientific line drawing to improve it?
- sketched lines ✓
- using pencil
- cell shape
- shading ✓
Which of these descriptions is not a key feature of an accurate scientific line drawing?
- labelling
- continuous, smooth lines
- stippling
- shading ✓
- magnification used

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The cells that make up tissue can be viewed using a light microscope.
A sample of tissue can be prepared on a slide to view using a microscope.
Adding a stain can make it easier to see some parts of cells.
Changing the magnification and focus of a light microscope enables stained cell structures to be observed.
Observations from a light microscope can be recorded in a scientific line drawing.

Producing a line drawing with sketched broken lines, shading, no labelling and no magnification.

Learning cycle 2 in this lesson explores how to produce a scientific line drawing to record observations from light microscopy.

Tissue - A tissue is a group of similar cells with the same job working together.
Slide - To observe a specimen using a light microscope, we have to put a thin layer of it on a glass microscope slide.
Stain - A coloured liquid put onto a specimen so that the cells and their structures can be more easily seen with a light microscope.
Scientific line drawing - Observations from a light microscope can be recorded by making a labelled scientific line drawing.