Starter quiz
- Match the variable to its description for the investigation 'Investigating the effect of changing pH on the rate of enzyme reaction'.
- pH⇔independent variable ✓
- enzyme amount⇔control variable ✓
- rate of reaction⇔dependent variable ✓
Is this a positive or negative test for starch?- positive ✓
- negative
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- Who best describes what rate of reaction is?
Sofia: How much substrate has been used up.
Alex: How much enzyme has been used.
Izzy: How much product was formed when the reaction finished.
Jacob: How much product was formed every second. ✓
- Order these steps to show how you would carry out an investigation into the effect of changing temperature on the rate of an enzyme-controlled reaction.
- 1⇔Measure 5 ml of starch and amylase solution into separate test tubes.
- 2⇔Place both test tubes in a water bath at 21 °C for five minutes.
- 3⇔Combine the solutions and mix gently. Return to the water bath.
- 4⇔Using a pipette, remove a sample of solution.
- 5⇔Test the solution in a spotting tile with iodine solution.
- 6⇔Continue testing every minute until the iodine solution stays orange/brown.
- 7⇔Repeat at different temperatures.
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